ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective effects of Shengui tablet (Chinese Traditional Medicine) on experimental cerebral ischemia by acute cerebral ischemia hypoxia in mice and bilateral ligation of the carotid artery in rats.</p><p><b>METHODS</b>In the acute cerebral ischemia hypoxia model, the mice were randomly divided into control group, low-, middle- and high-dose (0.16, 0.33 and 1.00 g/kg) groups of Shengui tablet, after oral treatment for 30 d, gasping time of isolated heads of mice were observed. In bilateral ligation of the carotid artery cerebral ischemia model, the rats were randomly divided into control group, model group and low-, middle-, high-dose (0.072, 0.149 and 0.450 g/kg) groups of Shengui tablet. After oral treatment for 7 d, the cerebral index, superoxide dismutase (SOD) activity and the content of malondialdehyde (MDA) were measured.</p><p><b>RESULTS</b>Compared with the control model, Shengui tablet middle- and high-dose could significantly prolong gasping time of isolate heads of mice. Compared with model group, Shengui tablet low-, middle- and high-dose could significantly decrease the cerebral index and enhance SOD activity in brain tissue; only high-dose could reduce the content of MDA.</p><p><b>CONCLUSION</b>Shengui tablet has significant protective effect on the cerebral ischemia.</p>
Subject(s)
Animals , Female , Male , Mice , Rats , Brain , Metabolism , Brain Ischemia , Metabolism , Drugs, Chinese Herbal , Pharmacology , Malondialdehyde , Metabolism , Mice, Inbred Strains , Neuroprotective Agents , Pharmacology , Rats, Sprague-Dawley , Superoxide Dismutase , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To construct an apparatus for the oxygen uptake measurement of rats exposed to hypobaric hypoxia at different simulated altitude.</p><p><b>METHODS</b>The capacity of this apparatus was about 0.01 m3. It included animal experimental cabin, reference cabin, altimeter, altitude vertical velocity indicator, pressure difference inductor and oxygen compensator, low scale manometer, soda lime and calcium chloride, small fan, thermometer, circulating water system and vacuum pump. The oxygen uptake of the rats at 6 000 m, 4 000 m and 1 000 m simulated altitude was measured using this apparatus.</p><p><b>RESULTS</b>The oxygen uptake of the rats at 50 m, 4 000 m and 6 000 m simulated altitude was (24.4 +/- 2.1), (10.8 +/- 2.0) and (8.8 +/- 1.6) ml O2/(kg x min) respectively (average +/- s, n = 10). The oxygen uptake decreased as altitude increased.</p><p><b>CONCLUSION</b>This apparatus can be used to measure the oxygen uptake of the rats at different simulated altitude.</p>